Cite Score:
0.25
ELSEVIER SCOPUS

Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls

AUTHORS

Sadia Rehman 1 , * , Nusrat Saba 1 , Naveed Akhtar 2 , Saeeda Munir 1 , Waqar Ahmed 3 , Azra Khanum 4

AUTHORS INFORMATION

1 Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan

2 Shifa College of Medicine, Shifa Tameer-e-Millat University, Islamabad, Pakistan

3 Armed Forces Institute of Cardiology and National Institute of Heart diseases, Rawalpindi, Pakistan

4 Pir Mehr Ali Shah Arid Agriculture University, Rawlpindi, Pakistan

How to Cite: Rehman S , Saba N , Akhtar N , Munir S , Ahmed W , et al. Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls, Int Cardio Res J. 2017 ; 9(3):e11238.

ARTICLE INFORMATION

International Cardiovascular Research Journal: 9 (3); e11238
Published Online: September 30, 2017
Article Type: Research Article
Received: February 28, 2017
Accepted: April 08, 2015

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: Valve scarring and collagen deposition are crucial in pathogenesis of Rheumatic Heart Disease (RHD), an autoimmune disorder of the heart. Angiotensin I-Converting Enzyme (ACE) plays a major role in fibrous tissue formation.

Objectives: The present research work aimed to assess the role of ACE Insertion/Deletion (I/D) polymorphism in progress of RHD.

Patients and Methods: DNA was pre pared from blood samples from 156 RHD patients (156) and 204 healthy ethnically-matched controls. Then, it was screened using sequence-specific Primers. Polymerase chain reaction and Agarose gel electrophoresis. The data were analyzed using Vassar stats (http://faculty.vassar.edu/lowry/VassarStats.html).

Results: I allele (P = 0.024, OR = 1.42) and II genotype (P = 0.001, OR = 3.07) were significantly higher in Pakistani RHD patients compared to the healthy controls. Also, a significant difference was found between the female, but not male, patients and the controls regarding I allele and II genotype.

Conclusions: The study results provided information about involvement of ACE I/D polymorphism in molecular mechanism of RHD. Thus, it can become one of the useful tools in risk assessment and help with designing strategies to combat the disease.

Keywords

Rheumatic Heart Disease ACE Polymorphism Pakistan

© 0, Shiraz University of Medical Sciences.

Fulltext

The full text is available in PDF.

References

  • 1. The references are available in PDF.
  • COMMENTS

    LEAVE A COMMENT HERE: