Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls
Article Notes and Dates
International Cardiovascular Research Journal: March 31, 2017,
; e11238.Published Online: September 30, 2017
Article Type: Research Article; Received: February 28, 2017; Accepted: April 08, 2015
To Cite :
S , Saba
N , Akhtar
N , Munir
S , Ahmed
W , et al. Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls,
Int Cardio Res J.
Copyright © 2017, Shiraz University of Medical Sciences. .
Background: Valve scarring and collagen deposition are crucial in pathogenesis of Rheumatic Heart Disease (RHD), an autoimmune disorder of the heart. Angiotensin I-Converting Enzyme (ACE) plays a major role in fibrous tissue formation.
Objectives: The present research work aimed to assess the role of ACE Insertion/Deletion (I/D) polymorphism in progress of RHD.
Patients and Methods: DNA was pre pared from blood samples from 156 RHD patients (156) and 204 healthy ethnically-matched controls. Then, it was screened using sequence-specific Primers. Polymerase chain reaction and Agarose gel electrophoresis. The data were analyzed using Vassar stats (http://faculty.vassar.edu/lowry/VassarStats.html).
Results: I allele (P = 0.024, OR = 1.42) and II genotype (P = 0.001, OR = 3.07) were significantly higher in Pakistani RHD patients compared to the healthy controls. Also, a significant difference was found between the female, but not male, patients and the controls regarding I allele and II genotype.
Conclusions: The study results provided information about involvement of ACE I/D polymorphism in molecular mechanism of RHD. Thus, it can become one of the useful tools in risk assessment and help with designing strategies to combat the disease.