Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran

AUTHORS

Farnoosh shateri 1 , Touraj Farazmandfar 1 , Ali Sharifian 2 , Reza Salehi Manzari 3 , Marzieh Attar 1 , Majid Shahbazi 4 , *

1 Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran

2 Department of Heart, Kosar Heart Center, Golestan University of Medical Sciences, Gorgan, Iran

3 Resident of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Arya Tina Gene (ATG) Biophamaceutical Company, Gorgan, Iran

How to Cite: shateri F , Farazmandfar T, Sharifian A , Salehi Manzari R , Attar M , et al. Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran, Int Cardio Res J. 2017 ; 11(3):e11025.

ARTICLE INFORMATION

International Cardiovascular Research Journal: 11 (3); e11025
Published Online: September 01, 2017
Article Type: Research Article
Received: August 21, 2017
Accepted: May 11, 2017

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Abstract

Background: The Coronary artery disease (CAD) occurs as a result of atherosclerotic
plaque formation. The interferon gamma (IFNγ) as a multifunctional cytokine is
involved in inflammatory processes in atherosclerosis.
Objectives: We investigated the relationship between IFNγ (+874T/A) SNP with CAD.
Moreover, we compared IFNγ mRNA expression in CAD patients and healthy controls.
Patients and Methods: This case-control study with randomized sampling included
300 patients with CAD and 301 normal controls. The SSP-PCR method was used for
genotyping IFNγ (+874T/A) gene polymorphism. Quantitative Real-Time PCR was
performed to measure IFNγ mRNA expression. All data was analyzed by GraphPad
software. The chi-square and ordinal logistic regression tests were used to analyze
differences in genotype frequencies.
Results: In this study, there was a significant association between male genders with
CAD (P < 0.001). There was a significant association between genotype T/T and Allele T
of IFNγ (+874T/A) polymorphism with CAD (P = 0.021 and P = 0.022, respectively). The
inheritance model analysis showed that two copy of allele T is required for increased risk
in CAD (P = 0.031). There was a significant association between the genotype T/T of IFNγ
(+874T/A) polymorphism with CAD patients with double and triple vessel disease (P =
0.030 and P = 0.013, respectively). The IFNγ mRNA level in CAD group was significantly
higher than control group (P = 0.024).
Conclusions: Conclusions: IFNγ gene functional polymorphism can be associated with
incidence and severity of CAD. IFNγ mRNA level was also increased in CAD patients
in comparison with controls. Therefore, IFNγ may play a role in predisposition to CAD.

Keywords

Interferon-Gamma Coronary Artery Disease Single Nucleotide Polymorphism

© 0, Shiraz University of Medical Sciences.

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